Table 3.
Synergistic effects of combination of TSH fraction and compounds 1 and 2 with acyclovir (ACV) on anti-HSV activity.
| Compounds Combination Ratio | 2 × IC50 | 1 × IC50 | 0.5 × IC50 |
|---|---|---|---|
| Experimental CI Values (Description—Graded Symbols) | |||
| ACV + TSH fraction | 0.113 | 1.033 | 1.253 |
| (++++) | (±) | (− −) | |
| ACV + Compound 1 | 0.745 | 1.21 | 1.243 |
| (++) | (− −) | (− −) | |
| ACV + Compound 2 | 0.102 | 1.169 | 1.202 |
| (++++) | (−) | (− −) | |
| Compound 1 + Compound 2 | 0.197 | 0.146 | 0.131 |
| (++++) | (++++) | (++++) | |
| ACV + Compound 1 + Compound 2 | 0.104 | 1.131 | 1.121 |
| (++++) | (−) | (−) | |
CI, combination index, a quantitative measure calculated by Calcusyn Software. This index quantifies the interaction between the tested compounds as described by Chou et al. [50]. In detail, CI from 0.1 to 0.3 means strong synergism (++++), 0.7 to 0.85 means moderate synergism (++), 0.9 to 1.1 means additive effect (±), 1.1 to 1.2 means slight antagonism (−), and 1.2 to 1.45 means moderate antagonism (− −). Obtained values represent the mean of three independent experiments.