Table 2.
TAG fatty acid profile and content in batch culture of P. lutheri supplemented with different initial bicarbonate concentrations.
| Bicarbonate (mM) | ||||||
|---|---|---|---|---|---|---|
| Before N-Limitation | After N-Limitation | |||||
| Fatty Acids (% TFA) | 2 | 9 | 18 | 2 | 9 | 18 |
| Saturated fatty acids | ||||||
| 14:0 | 11.2 ± 0.8 | 9.6 ± 0.9 | 9.6 ± 0.9 | 10.7 ± 0.6 | 9.7 ± 0.2 | 10.3 ± 0.4 |
| 16:0 | 22.6 ± 2.1 | 22.4 ± 1.3 | 22.4 ± 1.3 | 30.7 ± 0.8 | 27.4 ± 0.3 | 29.5 ± 0.5 |
| 18:0 | 5.6 ± 1.4 | 5.5 ± 1.7 | 5.5 ± 1.7 | 0.8 ± 0.1 | 0.9 ± 1.2 | 0.9 ± 0.2 |
| Sum of SFA | 39.5 ± 0.1 | 37.5 ± 3.9 | 37.5 ± 3.9 | 42.2 ± 1.4 | 38.1 ± 0.3 | 40.7 ± 1.4 |
| Monounsaturated fatty acids | ||||||
| 16:1 n-7 | 22.0 ± 3.3 | 22.9 ± 10.3 | 15.9 ± 0.3 | 36.5 ± 0.2 | 33.9 ± 0.1 | 30.8 ± 0.5 |
| 18:1 n-7 | 3.0 ± 0.9 | 4.5 ± 2.2 | 3.7 ± 0.5 | 2.0 ± 0.0 | 1.7 ± 0.1 | 1.8 ± 0.3 |
| 18:1 n-9 | 5.6 ± 2.1 | 4.5 ± 1.1 | 5.4 ± 0.8 | 2.1 ± 0.1 | 3.3 ± 0.2 | 2.4 ± 0.0 |
| Sum of MUFA | 30.7 ± 2.1 | 31.8 ± 7.1 | 24.9 ± 0.6 | 40.7 ± 0.2 | 38.9 ± 0.2 | 35.0 ± 0.2 |
| Polyunsaturated fatty acids | ||||||
| 18:2 n-6 | 3.5 ± 0.3 | 3.4 ± 0.3 | 3.9 ± 0.3 | 0.4 ± 0.1 | 0.4 ± 0.0 | 0.3 ± 0.0 |
| 18:3 n-6 | 0.2 ± 0.0 | 0.4 ± 0.0 | 0.8 ± 0.4 | 0.4 ± 0.0 | 0.6 ± 0.1 | 0.4 ± 0.1 |
| 18:3 n-3 | 1.2 ± 0.2 | 1.7 ± 0.2 | 2.0 ± 1.2 | 2.0 ± 0.0 | 2.4 ± 0.1 | 3.0 ± 0.2 |
| 18:4 n-3 | 1.5 ± 0.4 | 2.1 ± 0.3 | 1.7 ± 0.0 | 1.0 ± 0.1 | 1.5 ± 0.1 | 1.5 ± 0.1 |
| 20:4 n-6 | 1.3 ± 0.3 | 0.9 ± 0.2 | 1.6 ± 0.3 | 1.0 ± 0.1 | 1.1 ± 0.1 | 1.3 ± 0.1 |
| 20:5 n-3 | 7.6 ± 0.6 | 10.1 ± 1.4 | 10.8 ± 0.4 | 5.9 ± 0.5 | 7.9 ± 0.1 | 8.2 ± 0.5 |
| 22:5 n-3 | 0.4 ± 0.0 | 0.6 ± 0.0 | 0.7 ± 0.1 | 0.7 ± 0.1 | 1.1 ± 0.2 | 1.5 ± 0.1 |
| 22:6 n-3 | 3.5 ± 0.8 | 2.9 ± 0.1 | 1.4 ± 0.4 | 2.5 ± 0.5 | 4.2 ± 0.4 | 4.2 ± 0.3 |
| Sum of PUFA | 19.3 ± 1.4 | 22.1 ± 2.2 | 23.0 ± 1.6 | 13.9 ± 1.3 | 19.2 ± 0.6 | 20.6 ± 1.2 |
| Others | 10.6 ± 3.5 | 8.6 ± 1.0 | 16.9 ± 3.8 | 3.3 ± 0.3 | 3.8 ± 0.1 | 3.8 ± 0.1 |
| n-3 | 14.3 ± 1.9 | 17.5 ± 1.8 | 16.7 ± 1.8 | 10.4 ± 1.2 | 15.3 ± 0.8 | 15.9 ± 0.9 |
| n-6 | 5.0 ± 0.6 | 4.6 ± 0.4 | 6.3 ± 0.2 | 3.4 ± 0.1 | 3.9 ± 0.2 | 4.6 ± 0.3 |
| TAG (pg cell−1) | 0.9 ± 0.1 | 0.7 ± 0.2 | 0.8 ± 0.2 | 1.3 ± 0.1 | 2.4 ± 0.1 | 4.8 ± 0.1 |
| TAG (% TFA) | 42.9 ± 1.1 | 33.6 ± 6.1 | 28.5 ± 7.6 | 54.0 ± 1.6 | 68.3 ± 1.9 | 74.4 ± 6.4 |
Results are expressed as the mean ± SD of two replicates (n = 2). MUFA, monounsaturated fatty acids; PUFA, polyunsaturated fatty acids; SFA, saturated fatty acids; TFA, total fatty acids.