Table 4.
Tentative identification of Cp pigments based on chromatographic, UV-vis and polarity characteristics of HPLC peaks contained in the Cp Ethanol 2 extract flash chromatography fractions.
| Fraction | Peak | HPLC Rt (min) | λmax (nm) | Ratio | Tentative Identification | ||
|---|---|---|---|---|---|---|---|
| I | II | III | III/II | ||||
| F1 | 1 | 3.5 | 430.0 | 666.7 | - | Chlorophyllide a | |
| F2 | 2 | 9.3 | 408.8 | 666.7 | - | Chlorophyllone a | |
| F3 | 3 | 12.5 | 410.0 | 666.7 | - | Pheophorbide a | |
| F4 | 4 | 21.0 | 410.0 | 660.0 | - | Pyropheophorbide a | |
| 5 | 24.0 | 454.9 | 481.7 | 28% | Zeaxanthin | ||
| F5 | 6 | 26.4 | 447.6 | 474.4 | 46% | 5,6-epoxy β-cryptoxanthin | |
| 7 | 26.9 | 447.9 | 474.4 | 20% | β-cryptoxanthin | ||
| F6 | 8 | 34.7 | 429.4 | 666.7 | - | Chlorophyll a allomer | |
| 9 | 35.0 | 433.0 | 451.2 | 485.3 | 50% | Unidentified carotenoid | |
| 10 | 36.0 | 429.4 | 661.7 | - | Chlorophyll a | ||
| F7 | 11 | 36.8 | 430.6 | 663.0 | - | Chlorophyll a epimer | |
| 12 | 37.7 | 417.3 | 661.7 | - | DV-pheophytin a | ||
| 13 | 39.0 | 429.4 | 454.9 | 40% | Mutatochrome | ||
| 14 | 40.5 | 408.8 | 669.1 | - | MV-pheophytin a | ||
| 15 | 41.4 | 401.5 | 669.1 | - | Purpurin-7 phytyl ester | ||
| 16 | 42.3 | 410.0 | 666.7 | - | Pheophytin a | ||
| F8 | 17 | 43.6 | 454.9 | 480.4 | 23% | β-carotene | |