Table 5.
HRMS of commercially available standard pigments suspected in Cp flash chromatography fractions.
| Standardpigment | Formula | UPLC Rt (min) | Theoricalm/z | Experimentalm/z | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M. | [M + H]+ | [M + Na]+ | [M − Mg + 3H]+ | M. | [M + H]+ | [M + Na]+ | [M − Mg + 3H]+ | Fragments | |||||
| Chlorophyllide a | C35H34N4O5Mg | 7.08 | - | 615.2458 | - | 593.2764 | - | 615.2580 | - | 593.2761 | 533.2545 | - | - |
| Pheophorbide a | C35H36N4O5 | 7.05 | - | 593.2764 | - | - | - | 593.2762 | - | - | 533.2544 | 539.2417 | - |
| Pyropheophorbide a | C33H34N4O3 | 7.45 | - | 535.2709 | 557.2529 | - | - | 535.2699 | 557.2520 | - | 435.2537 | 256.3005 | - |
| Zeaxanthin | C40H56O2 | 8.14 | 568.4280 | - | - | - | 568.4268 | - | - | - | 549.4098 | - | - |
| β-Cryptoxanthin | C40H56O | 10.04 | - | 553.4409 | 575.4137 | - | - | 553.4346 | - | - | 533.4146 | 460.3685 | - |
| Chlorophyll a | C55H72N4O5Mg | 10.03 | - | 893.5432 | 915.5251 | 871.5737 | 893.5403 | 915.5237 | 871.5727 | 614.2376 | 555.2232 | 637.2258 | |
| β-Carotene | C40H56 | 10.83 | 536.4382 | - | - | - | 536.4370 | - | - | - | 444.3751 | - | - |