Table 5.
PSSM/Sift scores for CAD mutant lines showing category 1 brown midrib phenotype
| [EMS] | Family | No. Indiv 1/total* | Mutation (Nt) | Mutation (AA) | PSSM difference | Sift score |
|---|---|---|---|---|---|---|
| 0,3 |
645 |
3/3 |
G1734A |
E351K |
11.0 |
0.09 |
| |
759 |
1/3 |
G1218A |
V252I |
7.3 |
0.11 |
| |
823 |
1/5 |
G1026A |
G188R |
30.4 |
0.00 |
| |
1021 |
1/4 |
G990A |
G176R |
_ |
0.00 |
| |
1411 |
1/3 |
G936A |
A158T |
11.0 |
0.09 |
| |
1564 |
1/1 |
C1303T |
P280L |
_ |
0.00 |
| 0.75 |
814 |
1/1 |
C999T |
Q179Stop |
damaging |
damaging |
| |
877 |
1/3 |
C1079T |
H205Q |
8.0 |
0.01 |
| 884 | 1/2 | G1137A | G225R | 12.8 | 0.09 |
PSSM scores and Sift scores were obtained with PARSESNP and SIFT programs, respectively. Scores in bold text are predicted to be damaging for protein activity while scores in normal text are predicted to be without effect. Mutations with no scores result from the lack of alignment block for the given position. *Number individual plants showing category 1 phenotype/total number plants phenotyped per family.