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. 2013 Oct 15;13:159. doi: 10.1186/1471-2229-13-159

Table 7.

Primers used in TILLinG experiments

Primer 5′-3′ Primer sequence
CAD external Forward primer
5′-ACAGTTTGACCTGATGGAGCTCGAT-3′
CAD external Reverse primer
5′-GAAAACAAGTCAAATCGGACATAGG-3′
C3H external Forward primer
5′-ATATTTACCAACCGGACTAACCTTG-3′
C3H external Reverse primer
5′-AGTACAACACAATTCCAACTCTTCG-3′
CAD internal Forward primer with M13 tail
5′-CACGACGTTGTAAAACGACTTTCGGTCCATCATCG-3′
CAD internal Reverse primer with M13 tail
5′-GATAACAATTTCACACAGGTATGGGTCTTCTCTTC-3′
C3H internal Forward primer with M13 tail
5′-CACGACGTTGTAAAACGACACCACACTGAATTCGG-3′
C3H internal Reverse primer with M13 tail
5′-GATAACAATTTCACACAGGCATGTAAGTCACCAGT-3′
M13 Forward primer
5′-CACGACGTTGTAAAACGAC-3′
M13 Reverse primer 5′-GGATAACAATTTCACACAGG-3′

The M13 tail sequence is indicated in bold text.