Table 7.
Primer | 5′-3′ Primer sequence |
---|---|
CAD external Forward primer |
5′-ACAGTTTGACCTGATGGAGCTCGAT-3′ |
CAD external Reverse primer |
5′-GAAAACAAGTCAAATCGGACATAGG-3′ |
C3H external Forward primer |
5′-ATATTTACCAACCGGACTAACCTTG-3′ |
C3H external Reverse primer |
5′-AGTACAACACAATTCCAACTCTTCG-3′ |
CAD internal Forward primer with M13 tail |
5′-CACGACGTTGTAAAACGACTTTCGGTCCATCATCG-3′ |
CAD internal Reverse primer with M13 tail |
5′-GATAACAATTTCACACAGGTATGGGTCTTCTCTTC-3′ |
C3H internal Forward primer with M13 tail |
5′-CACGACGTTGTAAAACGACACCACACTGAATTCGG-3′ |
C3H internal Reverse primer with M13 tail |
5′-GATAACAATTTCACACAGGCATGTAAGTCACCAGT-3′ |
M13 Forward primer |
5′-CACGACGTTGTAAAACGAC-3′ |
M13 Reverse primer | 5′-GGATAACAATTTCACACAGG-3′ |
The M13 tail sequence is indicated in bold text.