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Left: Western blot analysis of P and DS secretomes. Densitometric quantification of vinculin and thrombospondin-1 using Quantity One 1D analysis software. Representative images: 10 μg of proteins were separated on SDS-PAGE and blotted on nitrocellulose membrane. Immunoblots were probed with antibodies against vinculin and thrombospondin-1. Right: ELISA assay for vitamin D binding protein expression in secretome samples (P and DS).
