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. 2013 Jul;8(3):244–256.

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© 2013 Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences

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High glucose increased the stability of the PA28-β protein in cultured retinal pericytes (PC). (A-B) Real-time polymerase chain reaction (PCR) showing that high glucose treatment of retinal PC and endothelial cells (EC) results in modest or no change in transcript levels for either PA28-a or PA28-β. NIH3T3 cells were used as control for treatment with high glucose and PCR analysis. (C) Chase assay using protein synthesis inhibitor, cycloheximide (CHX) showing that treatment with high glucose for 5 days increased PA28-β stability. (D) Quantification of the Western blot for PA28-β by normalizing the ratio of PA28-β signal intensity to β-Actin. The percent change for the signal is represented above each bar.