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. 2004 Apr 15;113(8):1210–1217. doi: 10.1172/JCI18195

Figure 1.

Figure 1

Immunogenicity of cochlin and β-tectorin peptides containing the KXXS MHC class II–binding motif. Female SWXJ mice were immunized with selected peptides containing the KXXS motif and derived from the sequences of inner ear–specific proteins. Eight to 10 days after immunization, LN cells were tested for recall proliferative responses to each peptide immunogen. (A) Coch 131–150 and β-tectorin 71–90 elicited marked dose-response reactivity compared with several other cochlin and β-tectorin peptides that were relatively nonimmunogenic. (B) ELISA analysis of 48-hour culture supernatants showed that recall responses to Coch 131–150 involved the proinflammatory Th1-like phenotype with elevated production of IFN-γ and virtually no production of IL-4. (C) Recall responses to β-tectorin 71–90 showed a similar Th1-like cytokine profile. Error bars show ± SE.