Skip to main content
. 2004 Apr 15;113(8):1149–1157. doi: 10.1172/JCI19478

Figure 1.

Figure 1

IGF-1 is overexpressed in eyes of transgenic mice. (A) RT-PCR confirmed that IGF-1 in the eye was a result of transgene expression. Pancreas mRNA was used as a positive control and liver mRNA as a negative control. The amplified band hybridized with an IGF-1 cDNA probe confirms the specificity of the RT-PCR. (B) Northern blot analysis was performed using samples from retina, retinal pigmented epithelium (RPE), choroid (Cho), sclera (Scl), and from the whole eye. Endogenous IGF-1 was detected only after long exposure (data not shown). (C) IGF-1 expression in transgenic retina by in situ hybridization. Sense probe did not give any signal. (D) In the transgenic retina, purple staining indicated expression of IGF-1 in the outer plexiform layer and in the inner segment of photoreceptors. No IGF-1 signal was observed in the retina of control mice. Con, control; GCL, ganglion cell layer; INL, inner nuclear layer; IS, inner segment of photoreceptors; L, lens; ONL, outer nuclear layer; OPL, outer plexiform layer; R, retina; Tg, transgenic. Scale bars: 72 μm.