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VEGF expression in the transgenic eye. (A) Total RNA was obtained from whole eyes and analyzed by Northern blot as indicated in Methods. Expression of VEGF transcripts was increased in 15-month-old transgenic mice. (B) Western blot analysis of VEGF protein in whole eye. Both 3- and 15-month-old transgenic mice showed a significant increase in VEGF protein. Actin documents equal loading.
