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. 2013 Oct 7;9:37. doi: 10.1186/1746-4811-9-37

Table 1.

Number and sequence validation of markers generated in the SH*RH population using the different profiling primers and restriction enzyme combinations

Primer AluI HaeIII MseI RsaI TaqI Markers (#) Tagged bin ranges (#) Sequenced markers (#) on target* sequences (#) on target**
bin ranges (#)
NBS1
 24
 40
 22
 50
 47
 183
 34
 37
 19
 8
NBS2
 51
 36
 56
 43
 50
 236
 29
 37
 14
 6
NBS5a6
 22
 33
 38
 35
 34
 162
 38
 82
 62
 14
NBS9
 22
 25
 18
 40
 46
 151
 29
 53
 13
 6
total NBS
 119
 134
 134
 168
 177
 732
 56
 209
 108
 23
NBS13R
 9
 7
 7
 22
 10
 55
 9
 34
 27
 5
NBS15F
 7
 15
 15
 34
 20
 91
 11
 33
 27
 4
TIR300F
 23
 16
 27
 27
 18
 111
 17
 65
 41
 6
TIR300Fc
 5
 26
 14
 18
 nd
 63
 9
 40
 32
 4
TIR3R
 16
 35
 11
 12
 15
 89
 12
 38
 20
 3
TIR9256
 13
 13
 29
 23
 13
 91
 13
 49
 22
 2
TIRWCF
 8
 17
 28
 47
 nd
 100
 17
 49
 26
 6
total N-like
 81
 129
 131
 183
 76
 600
 38
 308
 195
 12
chcF2
 14
 3
 9
 4
 nd
 30
 5
 5
 3
 0
chcR1
 5
 1
 12
 9
 nd
 27
 5
 9
 6
 2
chcR2
 8
 5
 9
 15
 nd
 37
 6
 16
 14
 3
total chc1-like
 27
 9
 30
 28
 0
 94
 9
 30
 23
 3
PK1Fa
 11
 9
 6
 16
 nd
 42
 12
 20
 13
 4
PK1Fb
 14
 10
 16
 9
 nd
 49
 16
 20
 11
 8
PK3Fb
 16
 14
 19
 26
 nd
 75
 16
 42
 11
 6
PK4R1a
 6
 12
 12
 10
 nd
 40
 13
 16
 4
 2
total PK
 47
 45
 53
 61
 0
 206
 35
 98
 39
 19
total 274 317 348 440 253 1632 69 645 365 43

* Confirmed as on target when the sequence had significant homology to known R genes or RGAs.

** Confirmed as on target if the tagged bin range contained at least one on target sequenced marker.

# = numbers of.