Figure 3.

Melanoma cell lines harboring modest expression of GLI2 and SMO were more sensitive to cyclopamine as compared to those cell lines with overexpression or absence of GLI2 and SMO. (A) Baseline levels of GLI1, GLI2, SMO, and PTCH1 were measured by qRT-PCR in four melanoma cell lines. (B) The A375 and WM3248 cell lines were sensitive to cyclopamine (IC₅₀ < 10 μM), whereas the WM278 and SkMel94 cell lines were resistant to cyclopamine (IC₅₀ > 45 μM). In A, values represent the mean ± standard deviation from a representative experiment performed three times with similar results. In B the values represent the mean from a representative experiment performed three times with similar results.