Figure 2.

Effects of testosterone-HSA on Rac1 GTPase in Caco2 cells. Caco2 cells were stimulated with 10⁻⁷ mol l⁻¹ testosterone-HSA for the indicated time periods. Following cell lysis equal amounts of total lysates were immunoblotted with a specific antibody anti-Rac1 and total protein. A positive control loaded with GTPγS was included in each assay. Immunoblots were analyzed by densitometry. The intensity of Rac1 GTPase was normalized to the intensity of the corresponding total protein band. Blots are from a representative experiment, whereas the relative fold increases are indicated as mean±s.e. from n=3 independent experiments of Rac1 GTPase with that of untreated cells (*P<0.05, **P<0.01). TAC, testosterone albumin conjugate.