Figure 3.

Modulation of the dynamic equilibrium between GST–PBD and total protein in testosterone-HSA-treated Caco2 cells in the presence or absence of wortmannin. Twenty-four-hour serum-starved cells were stimulated with 10⁻⁷ mol l⁻¹ testosterone-HSA in the presence or absence of 10⁻⁶ mol l⁻¹ wortmannin for the indicated time periods. Cellular content of GST–PBD versus total protein was measured by Western blotting using specific antibodies for each in cell lysates. Cells were pre-treated with wortmannin followed by testosterone-HSA stimulation. A positive control loaded with GTPγS was included in each assay. Representative blots from n=2 distinct experiments. GST–PBD, glutathione-S-transferase–p21-binding domain; TAC, testosterone albumin conjugate.