Figure 4.
Pro-apoptotic effects of testosterone-HSA in Caco2 cells. Caco2 cells stimulated with 10−7 mol l−1 testosterone conjugate for the indicated time periods. Quantitative APOPercentage apoptosis assay of testosterone-HSA stimulated Caco2 cells according to the manufacturer's instructions. Cells serum starved for comparable periods of time served as a positive control for apoptosis. Bars present the mean optical density (OD) measured at 550 nm from n=5 independent experiments. TAC, testosterone albumin conjugate.