Figure 2.

Expression of Lyzls in mouse epididymides following castration and subsequent testosterone manipulation. (a) RT-PCR detection of adult mouse epididymal RNA from males bilaterally castrated for 1, 3, 5 and 7 days (C1, C3, C5, C7), sham-operated males for 1 and 7 days (S1, S7) and 1, 3, 5 and 7 days after a single injection of testosterone propionate applied to 7-day castrated mice (C7+1, C7+3, C7+5 and C7+7) and sham-operated mice (S7+1, S7+3, S7+7 and S7+7). Total RNA was pooled from three animals at each time-point in castrated animals (b) and shams (c). The relative expression of Lyzls mRNA (expression of Lyzls mRNA/Gapdh mRNA) (in different colours and symbols) in the epididymides was compared with the serum testosterone level during androgen manipulation (n=3). Gapdh, glyceraldehyde-3-phosphate dehydrogenase.