Figure 3.

Expression of Lyzl6 gene and LYZL6 protein in the mouse testis and epididymis. (a) Total RNA isolated from the adult testis, caput, corpus and cauda was amplified by RT-PCR. (b) Western blot analysis of LYZL6 in the adult testis and epididymis. (c) Immunohistochemical localisation of LYZL6 in the adult mouse testis (c1), initial segment (c3), caput (c5), corpus (c7) and cauda (c9); c2, c4, c6, c8 and c10 are the corresponding negative controls. LYZL6 was mainly localized in the nuclei of primary spermatocytes (pri), round spermatids (rsd). The spermatogonia (spe) and elongated spermatids (esd) were all negative for LYZL6 staining. Basal (bas) and principal (prin) cells were positive in the epididymal sections. Scale bar=20 µm.