Table 1. Gene-specific primers used in this study.
Gene | Direction | 5′–3′ | Tm (°C)a | Cycles |
---|---|---|---|---|
Lyzl1 | ForwardReverse | GCATAGTCGCAGAATCCACAGCCCTGCCAATAGTTC | 51 | 28 |
Lyzl3 | ForwardReverse | GGCCAAGGTCTTCAGTCGACAGCCATCCACCCAGTC | 54 | 28 |
Lyzl4 | ForwardReverse | ACCAGAGCTTGGCGACGATGTGGCCGCACCATTCATT | 53 | 26 |
Lyzl6 | ForwardReverse | ATCCATCGCTGTAGTTTGTGAAATGAGGTTGGGTCT | 47 | 28 |
Gapdh | ForwardReverse | TCAACGGCACAGTCAAGGACCAGTGGATGCAGGGAT | 56 | 26 |
Abbreviation: Gapdh, glyceraldehyde-3-phosphate dehydrogenase.
Annealing temperature.