Figure 7.

Enhanced expression of TIMP-1 affects neither the intrahepatic recruitment of HBsAg-specific CTLs nor the intrahepatic expression of cytokines and chemokines. The recruitment of the passively transferred CTL lines was measured in the same livers described in the legend to Figure 3 by quantifying the amount of Sry-specific sequences and CCKAR-specific sequences by real-time PCR. The indicated numbers represent the average copy numbers of Sry-specific amplicons per 10,000 liver cell genomes (top). Total hepatic RNA from the same mice was also analyzed by RPA for the expression of various cytokines and chemokines as indicated (bottom). The housekeeping mRNAs encoding the ribosomal protein L32 or GAPDH were used to normalize the amount of RNA loaded in each lane.