Skip to main content
. 2013 Dec 5;9(12):e1003358. doi: 10.1371/journal.pcbi.1003358

Figure 2. Western blots for protein phosphorylation in stress-mediated BAD signaling pathway.

Figure 2

Both experiments were conducted in LNCaP cells. (A) Protein phosphorylation in cells treated with 50 µm LY294002 for 2 hours followed with increasing concentrations of epinephrine (0.01–1000 nm) for 1 h. BAD phosphorylation at Ser112 and CREB phosphorylated at Ser133 were measured. (B) Protein phosphorylation in cells treated with LY294002 (LY) followed with EGF 2 h later. Phospho-Ser473 Akt, phospho-Thr308 Akt, total Akt, phospho-ERK1/2, total ERK1/2, phospho-Ser112 BAD, and total BAD were measured for the indicated times. LY294002 inducing dephosphorylation of HA-BAD at Ser112 and Ser136 were also followed by Western blot analysis. Data from [6], [7]. Panel A reproduced from [7] and Panel B reproduced from [6] with permission from the American Society for Biochemistry and Molecular Biology.