Table 1. Primers used for gene amplification evaluated in white adipose tissue from sedentary and trained mice.

Genes	Sequence
Leptin	F: 5′-AACCCTCATCAAGACCATTGTCA-3′
	R: 5′-CCTCTGCTTGGCGGATACC-3′
Adiponectin	F: 5′-CGGCAGCACTGGCAAGTT-3′
	R: 5′-CCGTGATGTGGTAAGAGAAGTAGTAGA-3′
ADD1	F: 5′-GGAGCCATGGATTGCACATT-3′
	R: 5′-GGAAGTCACTGTCTTGGTTGTTGA-3′
FAS	F: 5′-TCCTGGAACGAGAACACGATCT-3′
	R: 5′-GAGACGTGTCACTCCTGGACTTG-3′
Malic enzyme	F: 5′-CTCCTTGTCCTTGGGCTCA-3′
	R: 5′-CACTACGCACAATCCGAGA-3′
HSL	F: 5′-TCTAGCATGGGGTCCAGAG-3′
	R: 5′-ACATGCCCCTCTACACGTC-3′
ALBP	F: 5′-GCGTGGAATTCGATGAAATCA-3′
	R: 5′-CCCGCCATCTAGGGTTATGA3′
Cyclophilin	F: 5′-AATGCGGACCAAACACAAA-3′
	R: 5′-CCTTCTTTCACCTTCCCAAA-3′

The primers were designed using the program Primer-Blast from NCBI-NIH (www.ncbi.nlm.nih.gov/tools/primer-blast/). F = forward; R = reverse; ADD1 = transcription factor adipocyte determination and differentiation-dependent factor 1; FAS = fatty acid synthase; HSL = hormone-sensitive lipase; ALBP = adipocyte lipid binding protein.