Figure 1. Representative retinal sections of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL), in which the fluorescein isothyocyanate (FITC)-12-dUTP-labeled fragmented DNA and nuclear counterstaining were performed using 4′,6-diamidino-2-phenylindole (DAPI). A, B, No treatment control; C, D, ocular ischemic injury only; E, F, agmatine treatment only; G, H, ocular ischemic injury after agmatine treatment. A, C, E, G, Blue fluorescence indicates DAPI; B, D, F, H, green fluorescence indicates FITC. The TUNEL-positive cells are emphasized as yellow circles in Panels B, F, and H, but all cells are TUNEL-positive in Panel D. Scale bar = 100 µM.
