Table 1. Effects of different concentrations of Ang II on [3H]-Leu incorporation, and mRNA levels of ANF and β-MHC in primary cardiomyocytes.
Ang II (µM) | [3H]-Leu incorporation (cpm/well) | Relative ANF mRNA | Relative β-MHC mRNA |
---|---|---|---|
Control | 1188.83 ± 72.20 | 1 ± 0 | 1 ± 0 |
0.01 | 1494.58 ± 65.00* | 1.07 ± 0.09 | 1.17 ± 0.06 |
0.1 | 1821.33 ± 54.65* | 1.20 ± 0.02* | 1.75 ± 0.03* |
1 | 2374.08 ± 49.34* | 1.44 ± 0.08* | 1.85 ± 0.13* |
10 | 2414.67 ± 56.70* | 1.65 ± 0.04* | 2.08 ± 0.14* |
Data reported as means ± SD for N = 6/concentration performed in triplicate. Ang II = angiotensin II; ANF = atrial natriuretic factor; β-MHC = β-myosin heavy chain.
P < 0.05 vs control (one-way ANOVA).