Figure 2. Western blot detection of neuronal nitric oxide synthase (nNOS) in hippocampi of WT and FMR1 KO mice at P7, P14, P21, and P28 (N = 3 per genotype, per age group). A, Representative blots of nNOS protein levels at each age in the hippocampi of the FMR1 KO and WT groups were compared to β-actin levels (loading control). B, Absorbance of nNOS immunoreactive bands normalized to their respective β-actin loading control from 3 animals per genotype per age group were averaged and are reported as means ± SEM. ^*P < 0.05 vs WT on P21 (two-tailed Student t-test). WT = wild-type mice; FMR1 KO = fragile X mental retardation 1 gene knockout mice; P7, P14, P21, P28 = postnatal days 7, 14, 21, and 28, respectively.