Figure 6. Consumption of GSH and production of GSSG. A, Pure GSH (100 µM); B, remaining concentration of GSH after the redox cycling reaction; C, production of GSSG after the redox cycling reaction. The reaction conditions were bromoquinone (10 µM) and GSH (100 µM) in PBS, pH 7.4, at 37°C. After 15 min, aliquots of the reaction mixtures were removed and submitted to derivatization and analysis. GSH = glutathione; GSSG = oxidized glutathione.