Figure 2. CM-Sepharose ion exchange chromatography (2.0 × 21.0 cm) of Albizia lebbeck seed coat proteins previously precipitated with ammonium sulfate (0-90% saturation) and dissolved in sodium acetate buffer, pH 5.0. The column was equilibrated and chromatography was developed with 0.1 M sodium acetate buffer, pH 5.0. The non-retained fraction was eluted with 0.1 M sodium acetate buffer, pH 5.0 (CI fraction) and adsorbed proteins were desorbed with 0.25 and 0.5 M NaCl (CII and CIII fractions, respectively).