Table 1. Mass spectrometry analysis of the peptides from the fraction of Albizia lebbeck seed coat obtained by Sephacryl S-100 gel filtration chromatography (SI fraction).
| Spot No. | Peptide sequence | Protein accession number and Blast bit score* | |
|---|---|---|---|
| Vigna radiata 8S globulin ABG02260 | Vigna unguiculata vicilin emb|CAP19902.1| | ||
| Band 1 | KQIQNLENYRV | 36.3 | 40.1 |
| RIPAGTTFFLVNPNDNDNLRI | 63.0 | 54.5 | |
| Band 3 | RWFHTLFRN | 36.3 | 40.1 |
| KIPAGTTFFLVNPDDNENLRI | 71.0 | 61.7 | |
| RAILTLVNPDGRD | 41.4 | 43.9 | |
| KQIQNLENYRV | 36.3 | 40.1 | |
| RGQNNPFYFDSDRW | 45.6 | 52.8 | |
| RLHEITPEKNPQLRD | 42.6 | 52.8 | |
| REQQQQQQEESWEVQRY | 53.2 | 62.2 | |
| KLAIPVNNPHRF | 40.1 | 40.1 | |
*
Bit scores were computed by aligning the MS peptides against the Vigna radiata 8S globulin protein ABG02260.1 (454 amino acids) and Vigna unguiculata vicilin protein (433 amino acids) using BLAST. Collectively, the peptides encompass approximately 24.7% of the V. radiata 8S protein and 25.9% of V. unguiculata vicilin. We used a 90% threshold for query coverage and 35 bit score (BLAST) to map the peptides on the vicilin and vicilin-like proteins.