Figure 4. 3-n-Butylphthalide (NBP) promoted the colocalization of α-synuclein and light chain 3 (LC3) in 1-methyl-4-phenylpyridinium (MPP+)-treated PC12 cells. The treated cells were immunostained with anti-α-synuclein (green) and anti-LC3 (red) antibodies. Nuclei were further stained with Hoechst 33342 (blue). Immunoreactivity was visualized under a confocal laser scanning microscope. Scale bars = 37.52 µm.
