Figure 4. Cell-cell interaction is crucial for maturation induction of immature dendritic cells (iDCs) by apoptotic MCF-7 cells. The co-culture of iDCs with doxorubicin (ADM)-treated MCF-7 cells was performed either together in the same compartment or separated by 0.4-µM pore-sized filter Transwell inserts on a 24-well culture plate for 24 h. Then, the CD11c-positive DC population was gated and the DC-surface expression of CD83, CD86 and HLA-DR was analyzed by FACS. Data are reported as mean fluorescence intensity (MFI) ± SD for three independent experiments. ^*P < 0.05 vs Transwell co-culture group (one-way ANOVA).