Figure 2. Suppression effects of Ad-siTNFα-BMP-2 on titanium particle-induced inflammatory factor. A, RAW264.7 cells were plated on 6-well cluster plates at a density of 4×105 cells/well. After 24 h, RAW264.7 cells were treated with or without Ti particles in the presence or absence of Ad-siTNFα-BMP-2 for 48 h. There was a significant reduction of TNF-α mRNA expression at 50 MOI . B, TNF-α protein levels detected by Western blot after 48 h. There was significant reduction of IL-6 (C) and IL-1β (D) mRNA expression by Ad-siTNFα-BMP-2 at 50 MOI. E, TNF-α protein levels detected by ELISA when treated with 0.1 mg/mL Ti particles or a combination of 0.1 mg/mL Ti particles and 50 MOI Ad-siTNFα-BMP-2 for 24 h. Data are reported as means±SD. Similar results were obtained in three independent experiments. MOI: multiplicity of infection. *P<0.05, compared to the cultures with Ti particles only; +P<0.05, compared to 30 MOI (one-way ANOVA).
