Figure 3. Effect of treatment with butyrate (BT) and/or vitamin A (VA) on H3K9 acetylation. Western blot analysis of H3K9 acetylation for MCF-7 cells treated or not with BT and VA, individually or combined, for 96 (A) and 120 h (B). Quantitation of histone H3K9 acetylation in MCF-7 cells treated or not with BT and VA, individually or combined, for 96 (C) and 120 h (D). The data are representative of three independent experiments and are reported as means ± SEM. ^aP ≤ 0.5 compared to control; ^bP ≤ 0.5 compared to treatment with VA (ANOVA followed by the Tukey test).