Figure 4. Effect of treatment with butyrate (BT) and/or vitamin A (VA) on p21WAF expression. Western blot analysis of p21WAF expression was carried out in MCF-7 cells treated or not with BT and VA, individually or combined, for 96 (A) and 120 h (B). Quantitation of p21WAF expression in MCF-7 cells treated or not with BT and VA, individually or combined, for 96 (C) and 120 h (D). The data are representative of four independent experiments and are reported as means ± SEM. aP ≤ 0.5 compared to control; bP ≤ 0.5 compared to treatment with VA (ANOVA followed by the Duncan test).
