Figure 5. Effect of treatment with butyrate (BT) and/or vitamin A (VA) on global DNA methylation. Dot-blot analyses for global DNA methylation patterns were determined in MCF-7 cells treated or not with BT and VA, individually or combined, for 96 (A) and 120 h (B). Membrane staining with methylene blue to control for unequal loading of total DNA of MCF-7 cells treated or not with BT and VA, individually or combined, for 96 (C) and 120 h (D). Quantitation of DNA methylation patterns in MCF-7 cells treated or not with BT and VA, individually or combined, for 96 (E) and 120 h (F). The data are representative of three independent experiments conducted in duplicate and are reported as means ± SEM. No statistically significant differences were detected according to the Kruskal Wallis test (P > 0.05).
