Figure 7. Effect of treatment with butyrate (BT) and/or vitamin A (VA) on RARβ promoter methylation. MS-PCR analysis of RARβ promoter methylation was carried out in MCF-7 cells treated or not with BT and VA, individually or combined, for 96 h. The presence of the “U” product indicates the unmethylated RARβ gene. The presence of the “M” product indicates the methylated RARβ gene. H2O served as the negative control.
