Figure 2. Human cardiac troponin I-interacting kinase (TNNI3K) phosphorylates cardiac troponin I (cTnI) at Ser43 (S43) and Thr143 (T143). HEK293T cells were transfected with Xpress-tagged TNNI3K, Xpress-tagged TNNI3Kmut, or Flag-tagged cTnI and incubated for 72 h. A, Western blot using anti-Flag and anti-Xpress antibodies (n = 5). B, Western blot using anti-Xpress and anti-p-cTnI antibodies (n = 5). C, Western blot using anti-P-cTnI (S43) and anti-Xpress antibodies (n = 5). D, Western blot using anti-P-cTnI (Thr143) and anti-Xpress antibodies (n = 5). IP = immunoprecipitated; IB = immunoblotted. Data are reported as means ± SE. *P < 0.01 vs TNNI3Kmut-mediated phosphorylation (one-way ANOVA).
