Figure 4. Knockdown of human cardiac troponin I-interacting kinase (TNNI3K) in isolated rat adult cardiomyocytes. Freshly isolated rat adult cardiomyocytes were infected with an adenovirus carrying shRNA for silencing TNNI3K or scrambling shRNA for 24 h at the indicated multiplicity of infection (MOI). Samples were analyzed by quantitative real-time reverse transcription polymerase chain reaction and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data are reported as means ± SE (n = 6). *P < 0.01 vs control (MOI = 0); #P < 0.01 vs green bar at the same MOI value (one-way ANOVA).
