Figure 7. Effect of treatment with 5azadC (1, 10 and 100 µm) on differentiation of MSCs. A, Effect of 5azadC on the adipogenic differentiation of BM-MSC and ADSC cultures observed after staining with Oil Red O. B, Relative quantification of adipocyte differentiation. C, PPARG and D, FABP4 expression in the cultures after 14 days of adipocyte differentiation. 5azadC = 5-aza-2′-deoxycytidine; MSCs = mesenchymal stem cells; BM-MSCs = bone marrow MSCs; ADSCs = adipose tissue stem cells. Data are reported as means ± SD. Asterisks just above SD bars indicate statistically significant differences with respect to untreated cultures of BM-MSCs or ADSCs. *P < 0.05 (Student t-test).
