Figure 2. Time required for induction of anesthesia in silver catfish with globulol in association with benzodiazepine (BDZ): stage 2 (A), stage 3a (B) and stage 3b (C) according to Schoettger and Julin (22), and recovery time (D). Maximum observation time was 30 min to induction and 60 min to recovery. Time to reach each stage is given in seconds (s). Data are reported as means±SE (n=8). *Significantly different compared to BDZ; different uppercase letters indicate significant differences between globulol and association (globulol+BDZ) at the same concentration; different lowercase letters indicate significant differences between concentrations of the same group. Two-way ANOVA and the Tukey test or Scheirer-Ray-Hare extension of the Kruskal-Wallis test and the Dunn test were used (P<0.05). The control group of vehicle treatment is omitted because it did not produce any stage of anesthesia.