Figure 13. Concentration-dependent inactivation of human angiotensin-1 converting enzyme (sACE-1) by Fe-NTA-lisinopril (17). A, sACE-1 (1 nM) was preincubated with variable concentrations of Fe-NTA-lisinopril, and subsequently sACE-1 activity was measured from the initial cleavage rates of fluorogenic substrate. B, Dependence of sACE-1 activity on Fe-NTA-lisinopril concentration. IC50 values were determined for all synthesized M-chelate-lisinopril complexes in the same manner.
