Figure 16. Time-dependent inactivation (A) and cleavage (B) of full length sACE-1 by Cu-GGH-lisinopril with the coreactants 1 mM ascorbate and 1 mM H2O2 (circles), 1 mM ascorbate (triangles), 1 mM H2O2 (inverted triangles), or no coreactant (lozenges) (17). Time-dependent cleavage of sACE-1 was quantified by SDS-PAGE (C), shown here for the reaction of Cu-GGH-lisinopril with ascorbate and H2O2.
