FIGURE 1.

Dimerization of the 5′ UTR in the presence of DNA oligomers annealed to different regions of this RNA. (A) The wild-type 5′-UTR RNA (the first 406 nt of gRNA) was annealed with different DNA oligomers: cPBS(182–199), complementary to the PBS; cGAG(369–386), complementary to a coding sequence of Gag; cDIS(257–280) and cDIS(236–254), complementary to two different regions of the DIS hairpin. Monomeric and dimeric 5′-UTR RNA were resolved by electrophoresis in agarose gels, and detected by EtBr staining. (B) 5′-UTR RNA dimerization in presence or absence of cPBS(182–199) in dimerization buffer using varying concentrations of KCl.