FIGURE 3.

XRNE depletion results in a modest growth phenotype in T. brucei. T. brucei cells harboring tet-inducible RNAi constructs were induced or left uninduced, and growth of these and tet-treated 29-13 parental cells was monitored for 10 d. (Inset) Quantitative RT-PCR demonstrating the relative abundance of XRNE mRNA from the tet-induced and uninduced RNAi cell lines compared to tet-treated 29-13 parental cells. A value of 1 indicates no change in transcript compared to 29-13 cells, whereas negative values indicate the level of depletion of XRNE mRNA. Error bars indicate standard deviation of triplicate determinations.