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. 2013 Oct;19(10):1419–1431. doi: 10.1261/rna.038547.113

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© 2013; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 5. — 5′ extended 5.8S rRNA can integrate into ribosomes. Northern blot analysis of RNA isolated from fractions obtained from sucrose gradient fractionation (10%–40%) of induced XRNE RNAi and 29-13 parental cells, with fraction number shown across the top of each set of panels. Lanes indicated with a “C” contain total RNA from tet-induced XRNE RNAi cells and are used as a marker for sizes of aberrantly processed rRNAs. (A) Northern blot analysis of gradient fractions using pre-18S probe. (B) Northern blot analysis of select fractions using the pre-5.8S probe. Fractions corresponding to peaks containing 40S (SSU) and 60S (LSU) subunits, 80S monosomes, and polysomes are indicated. Bottom panels in both A and B are images of the methylene blue staining of the region of the membranes where SSU and two largest LSU rRNAs are located and demonstrate accuracy of fractionation.