Figure 3. GCMS analysis of products formed in vitro with SaCYP76F39v1.

A sesquiterpene mixture of α-, β- and epi-β-santalene and α-exo-bergamotene (Figure S3) was incubated with microsomes containing SaCYP76F39v1 and SaCPR produced in yeast. (A) Product profile (extracted ion chromatogram, EIC) of assays with SaCYP76F39v1. (B) Authentic S. album oil. (C) Control assays were performed with microsomes isolated from yeast cells transformed with the empty vector. Mass spectra of compounds corresponding to peaks 5–12 identified in assays with SaCYP76F39v1 (left panel) and S. album oil (right panel) are shown in Figure S4. Peak numbers match the numbers in Table 1 and Figure 1.