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. 2013 Oct 15;4(5):125. doi: 10.1186/scrt336

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Copyright © 2013 Ribeiro et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cells displaying increased forward scatter and side scatter light dispersion properties correspond to proliferating cells. Left: Bivariate dot plot histogram illustrating mononuclear cell (MNC) culture after phytohemagglutinin (PHA) stimulation, displaying two distinct cell populations: A) MNC with low forward scatter (FSC) and side scatter (SSC) light dispersion properties and B) MNC with increased FSC and SSC properties. Right: Density histogram illustrating the DNA content of MNC from population A (green) and B (red), measured after propidium iodide staining, and proving that cells with increased FSC and SSC are actively proliferating.