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. 2013 Jul 4;4(4):76. doi: 10.1186/scrt227

Figure 7.

Figure 7

HUMSC-NC transplantation increased the expression of Aβ-degrading enzymes NEP and IDE. (A, B) The mRNA levels of NEP (A) and IDE (B) in the cortex or hippocampus were determined with RT-PCR. Both NEP and IDE mRNA levels were increased significantly in the cortex and hippocampus of HUMSC-NC-treated mice. (C) Representative Western blots for NEP and IDE protein expression in the cortex or hippocampus: 100 μg of protein was load in each lane. Duplicate samples of each group were loaded into the gel. (D, E) Quantification of the Western blot for NEP (D) and IDE (E). The densitometry of NEP and IDE bands were first normalized to the loading control β-actin. The percentage of the expressions of NEP and IDE in the HUMSC- or HUMSC-NC-treated group relative to that in the PBS-treated group was then calculated. The scanned image of Western blot was analyzed with the software Image J. Data were presented as mean ± SEM. *P < 0.05, HUMSC-NC-treated group versus PBS-treated group.