Figure 3.
Polybrene negatively impacts the proliferation and maintenance of growth potential of human keratinocytes. (A) The effects of polybrene on keratinocyte proliferation are shown. Keratinocytes (2 × 105 cells) were seeded and treated with various concentration of polybrene overnight, and then the number of keratinocytes were counted after seven days of cultivation. The values (means ± SD) were determined based on results in triplicate experiments. (B) Determination of colony-forming efficiency (CFE) of keratinocytes cultivated with various concentrations of polybrene is shown. After seven days of cultivation with the condition described above, 104 cells were passaged and cultured without polybrene for eight days until the cultures were fixed and stained with rhodamine B. Bar, 10 mm.