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. 2013 Oct 30;4(5):133. doi: 10.1186/scrt344

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Copyright © 2013 Rutigliano et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Staining of differentiated and control undifferentiated feline AECs and respective molecular expression. A) von Kossa staining after osteogenic induction and RT-PCR analysis of osteopontin (OPN) and osteocalcin (OCN). B) Oil Red-O positive cytoplasmic neutral lipids after adipogenic induction and RT-PCR analysis of PPAR-γ and adiponectin (ADPQ). C) Alcian blue staining after chondrogenic induction and RT-PCR of aggrecan (ACAN) and collagenase (COL2A1). D) Nissl staining after neurogenic induction and RT-PCR of nestin. Magnification 20×; scale bar = 20 μm. GAPDH was employed as a reference gene. Bone, adipose tissue, cartilage and spinal cord were used as positive controls. AECs, amniotic epithelial cells.