Table 1.

Oligonucleotide sequences used for RT-PCR analysis

	Gene	Primers	Product size
Housekeeping gene	GAPDH	Forward, 5′ – ACGATGACATCAAGAAGGTG – 3′	180 bp
		Reverse, 5′ – CATACCAGGAAATGAGCTTG – 3′
Pluripotent markers	Oct4	Forward, 5′ – GGAGTCCCAGGACATCAAAG – 3′	285 bp
		Reverse, 5′ – GCCTGCACAAGTGTCTCTGC – 3′
	Nanog	Forward, 5′ – ACGGATCCAGCTCAGCCCCA – 3′	192 bp
		Reverse, 5′ – GGGGCTGCCCTGAGCAAGTA – 3′
Mesenchymal markers	CD44	Forward, 5′ – TGGGTTGTTTGGCATCCAGTGC – 3′	100 bp
		Reverse, 5′ – CGTTTTCTTCAGTTGGTTCCCAGCC – 3′
	CD166	Forward, 5′ – ACTGGCAGTGGAAGCGTCAT – 3′	275 bp
		Reverse, 5′ – CAGCAAGGAGGAGACCA – 3′
	CD29	Forward, 5′ – GGAAACTTGGTGGCATTGTT – 3′	180 bp
		Reverse, 5′ – GTTCCTTGTAAACGGGCTGA – 3′
	CD73	Forward, 5′ – AGCAAAGGGGCCACTAGCATCT – 3′	233 bp
		Reverse, 5′ – ACCCGAATGTCCCAGTGCAA – 3′
	CD90	Forward, 5′ – GAGCACACGTACCGCTCCCG – 3′	233 bp
		Reverse, 5′ – AGCAGCAGCAGCAGCATCCA – 3′
Hematopoietic marker	CD34	Forward, 5′ – CTTTAACTGTCACGGCGTTT – 3′	198 bp
		Reverse, 5′ – TGACTCGGGAACATTTGATT – 3′
Immunological markers	MHC-I	Forward, 5′ – CATCACCCTGAGATGGGAGC – 3′	176 bp
		Reverse, 5′ – TGGGTACTGTCGTCGCGTG – 3′
	MHC-II	Forward, 5′ – TCCGGAATCAGAAAGGACAC – 3′	172 bp
		Reverse, 5′ – GGCAAACCAAATCCTGAGAA – 3′
Osteogenic markers	OCN	Forward, 5′ – CTGCCTCTGCCTGGCTGGTC – 3′	120 bp
		Reverse,5′ – TAGCGCCGGAGCCTCCTCAC – 3′
	OPN	Forward, 5′ – ACTGGTCACTGATTTTCCCACGGA – 3′	100 bp
		Reverse, 5′– AACCACACTATCACCTCGGCCA – 3′
Adipogenic markers	ADPQ	Forward, 5′ – TGAGAAAGGAGATCCAGGTC – 3′	308 bp
		Reverse, 5′ – TCAAGTAGACTGTGATGTGG – 3′
	PPAR-γ	Forward, 5′ – CATGGTTGACACAGAGATGC – 3′	239 bp
		Reverse, 5′ – GCTCCACTTTGATTGCACTTTG –3′
Chondrogenic markers	ACAN	Forward, 5′ – AAGTGGAGCCGCGTTTCCAAGG – 3′	163 bp
		Reverse, 5′ – AGTCATTGGAGCGCAGGTTCTGG – 3′
	COL2A1	Forward, 5′ – AGTTGGGAGTAATGCAAG – 3′	294 bp
		Reverse, 5′ – GATAACCTCTGTGACCTTTG – 3′
Neurogenic marker	NES	Forward, 5′ – AAACAGGGCCTACAGAG – 3′	293 bp
		Reverse, 5′ – ACAGGTGTCTCAAGGGTAG – 3′